We studied the diet of two pairs of Eurasian Eagle Owl Bubo bubo in Castellar and Gorbio (Alpes-Maritimes), according to prey remains collected in the nests. 411 preys, distributed in 51 species, were identified. In Castellar the Eagle Owls’ diet was based mainly on Brown Rat Rattus norvegicus (37%), Black Rat Rattus rattus (24%) and Edible Dormouse Myoxus glis (12%). In Gorbio the main preys were Black Rat (38%), Brown Rat (14%), Hedgehog Erinaceus europaeus (9%) and Feral Pigeon Columba livia dom. (6%). Our data show the Eagle Owls we studied captured most of their prey in highly anthropized habitats (villages and dumps).